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Genopattern Analyzer
GP1000 |
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GP1000 has been discontinued.
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SIMPLIFY ANNOYING IDENTIFICATION OF BACTERIUM AND VIRUSES. CATCH ACCURATE DATA AT GENE LEVEL RIGHT NOW. |
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- Possible to identify each bacterium and viruses in the shortest 30 minutes (*1) by the pattern analysis of "DNA Dissociation Wave" -
This is the newest analyzer using GENOPATTERN method, which is developed from the creative imagination. The base sequence of bacterium, viruses and all others is to be analyzed.
(*1) The reaction time of a single-stranded DNA extension depends on samples or conditions. |
 Features |
- No need to extract DNA samples - Sequential operation is possible from the reaction of a single-strand extension to the pattern analysis of wave.
- Everybody easily operates and uses the analyzer, which makes their operation errors reduced.
- No genome extraction is required, because test specimens (microbes, cells) become directly the test sample to be analyzed. (*2)
- High accurate temperature distribution is available over all well (48 samples) with individual zone temperature controller.
- Accurate wave pattern is obtained with step less temperature controller and rapid data scanning.
- Genera and species of bacteria and virus are classified and identified very easily by "Geno Master". In addition, the master wave pattern can be registered.
- Most genes of animals, plants, microbes can be analyzed through the primer that is produced with the software "DESIGN SUPPORT TOOL FOR GENOPATTERN PRIMER" (*3).
- Available for a broad varieties of applications such as the inspection of infectious diseases to domestic animals & pets, the analysis of food allergy caused by food genes, the group expression of adverse drug reactions, the diagnosis of body predisposition or so.
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| (*2)All specimen might not become the test sample.. (*3) Optional accessory at extract cost. |
| PRINCIPAL OF GENOPATTERN METHOD |
DNA strand is annealed with a primer for Genopattern on its multiple sites to generate multiple single-strand nucleic acids containing base sequences downstream of distinct sites, which are associated with the primer and distributed throughout the whole strand. These nucleic acid products interact with one another to form complexes consisting of a number of products. As the temperature is raised, these complexes become progressively dissociated and denatured. The process of this dissociation and denaturation is observed fluorometrically and visualized as a wave pattern by graphically plotting the rate of the change of fluorescence intensity vs. temperature.
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| MEASUREMENT FLOW |
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1. Input the program data of the temperature and time, which are necessary to the wave pattern analysis and the reaction number of single-strand extension.
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3. Set samples
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2. Put the sample and reagent into sample tubes.
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4. Start
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5. Preparation
process / wave pattern analysis / preparation process for wave pattern
analysis: single-strand DNA extension reaction
(approximately 30 min. to 2 hours).
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6. Identify bacterium or viruses by pattern analysis software (Geno Master).
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| Specifications |
| Product Code |
317110 |
| Model |
GP1000 |
| Temperature Setting range |
0-100deg.C |
| Temperature Rising Speed |
1.6deg.C/sec |
| Temperature Falling Speed |
1.4deg.C/sec |
| Temperature Rising/Falling Method |
Peltier modules |
| Number of Sample·Tube Capacity |
48 samples·200µL |
| Sample Capacity |
50µL |
| Light Source |
Blue LED |
| Photo Detector |
Photo Multiplier |
| Power Source |
AC100V 700W |
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Consists of System
1. Reaction Chamber (Temperature control system + Optical measurement system)
2. PC/Interface
3. Data Control・Power Supply Box
Accessories
1. GP1000 tube (0.2ml, 50 ea)
2. Genopattern wave pattern Master Library-Geno Library (sample data・CD-ROM) |
| Dimensions |
 (mm) |
| GP1000 Reagent Test Kit |
| Product Code |
317111 |
317112 |
| Product Name |
Universal reagent for bacteria Gene research by Genopattern: Adgene
Geno-Universal Kit |
Acid Fast bacteria detection reagent for bacterial gene research
by Genopattern:Adgene Geno-Myco Kit |
| Content Volume |
For 20 times |
For 20 times |
| Delivery Conditions |
Freezed |
Freezed |
| Storage Conditions |
-20—30deg.C at dark place |
-20—30deg.C at dark place |
| Number of Freeze-thaw |
Within 8 times |
Within 8 times |
| Detectable Bacterium |
Bacterium extracted from DNA sample, suspended in water· saline |
Bacterium extracted DNA sample |
| Wave Pattern Detectable Bacterium |
All bacteria |
Mycobacterium genera |
| Bacterium Possible to be Identified |
Depends on the master data. |
Depends on the master data. |
| Necessary Amount of Sample |
Over 300 ng/ tube of DNA,over 106 ea/tube of bacterial |
Over 300 ng/ tube of DNA |
| Sample Preparation |
DNA extraction (Some bacterium do not need) |
DNA extraction |
| Kit Contents |
1. Genopattern Primer
2. GP1000 Buffer Mix
3. Synthetic Enzyme
(1&2 are absolutely necessary for Genopattern reaction as Adgene
original components) |
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Examples of bacterium samples possible to be identified by Universal reagent for bacteria Gene research by Genopattern:
Vibrio parahaemolyticus/ Pseudomonas aeruginosa/
Salmonellar typhimurium/ Enteroco/ Klebsiella pneumoniae/ Campyrobacter
jejuni/ Shigella sonnei/ ccus faecalis/ Haemophilus influenzae/ Helicobacter
pylori/ Staphylococcus pyogenes/ Mycobacterium bovis/ Escherichia
coli/ Bacillus cereus/ Staphylococcus aureus/ Bacillus subtilis,
etc…
(Master Data Library is to be enhanced and enriched upon available.)
- The above kit is for research use only.
- We have "economy kit" without synthetic enzyme. When using "economy kit", you also need separate synthetic enzyme.
- "Genopattern Buffer Mix" should be used, when using DESIGN SUPPORT TOOL FOR GENOPATTERN PRIMER.
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Reagent List
| Product Code |
Product Name |
| 317111 |
Universal reagent for bacteria Gene research by Genopattern: Adgene
Geno-Universal Kit |
| 317112 |
Acid Fast bacteria detection reagent for bacterial gene research
by Genopattern: Adgene Geno-Myco Kit |
| 317113 |
Universal reagent for bacteria Gene research y Genopattern: Adgene
Geno-Universal Kit, Economy Kit |
| 317114 |
Acid Fast bacteria detection reagent for bacterial gene research
by Genopattern: Adgene Geno-Myco Kit, Economy Kit |
| 317115 |
Genopattern Buffer Mix (For Primer Design) |
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Adgene Geno-Universal Kit |
| Other Optional Accessories |
Design Support Tool for Genopattern Primer
This design support tool helps your own design-making of Genopattern Primer freely. For details, please contact us |
Wave Pattern Master Data Library of Genopattern Method - Geno Library
We are going to enhance & enrich a variety of wave pattern master data library in order to support your researches. |
Wave Pattern Example (Acid Fast Bacteria)
The following wave patterns are a few examples that GP1000 can analyze. |
Note: The above graphic wave patterns are image samples. |
<For reference> Application to Genopattern method
Genopattern-RNA Method
The expression balance of subjective RNA-induced by drugs, is easy compared with the wave pattern.
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